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Services
Technical Support & Design Advice
IDC's technical services can assist you with your particular
project, and provide you with protocols for a wide range of
applications.
Custom Products and R&D
The members of our technical staff have considerable experience
in the use and selection of latex particles. We can prepare
custom latexes with size, mechanical, and surface properties
tailored to your specific needs. If you need help developing
new applications, please contact us. We have solved a variety
of problems for our customers in the areas of macromolecular
(protein) absorption, covalent coupling of ligands to latexes,
particle agglutination, ion exchange, and optical behavior of
latexes. Whether you are a seasoned veteran or a newcomer to
latexes, IDC is here to help you develop your projects properly
and efficiently.
Custom Latex Characterization
IDC can provide a quick and reliable physicochemical characterization
of polymer microspheres in our laboratories. Maintenance of
consistent latex particle performance in medical diagnostics
and other applications can be a costly problem. The custom characterization
services offered by IDC provide basic descriptive and quality
control data on particle properties which influence the behavior
of latexes and dictate how they should be handled in manufacturing
processes. These services are confidentially tailored by our
staff to meet your special needs. The services offered include
determinations of particle size, particle charge, specific surface
area, and stability of particles towards electrolytes and other
agents. Characterization services include:
Conductometric Titration
The surface charge of latex particles is determined by direct
titration using conductivity changes in the suspending medium
to determine the equivalence point. Experimental data from the
procedure are used to calculate the charge density on the surface
of the latex particle in µC/cm2, micro-equivalents
per gram or per charge group (Å2/charge group).
Conductometric Titration, per titration - $150
Mixed-bed Ion Exchange Clean Up
This procedure converts all acid end groups of polymer chains
to the hydrogen form and removes ionic surfactant if present.
Per item cost for 100ml - $150
Particle Size Analysis by Transmission Electron Microscopy
The mean diameter of the particle population and the percent
coefficient of variation of the diameter of the particles are
provided based on measurements of about 500 particles or until
further counting does not change the distribution. The measurements
also tell whether the particle population is monodisperse (uniform),
polydisperse, or multimodal.
TEM particle size analysis, per analysis - $300
TEM size analysis traceable to NIST standards - $500
Latex Handling
Lectures
IDC's staff and resources are available for in-house training
at your location or ours. We can give you lecture presentations
with information on the best way to work with our colloidal
products.
The Lab Companion
Designed to fit snuggly into the pocket of your lab coat, this
little volume will always be on hand to help. Inside you will
find out what makes up a latex particle, why they are colloidally
stable and what effects their sedimentation behavior. The choice
of particle for different types of assay is discussed in terms
of particle size and type active surface. Our suggested protocols
are described in detail with the composition of the buffer systems.
Finally there is a list of useful formulae which you may need.
It is printed so that it will stay open on the bench and we
have used lacquered paper so that you can wipe away any spillage
without harm.
Free while supplies last.
Include
one with your next order.
Selected Publications Which Cite The Use Of IDC Latexes
Elimelech, M. and O'Melia, C.R. 1990. Effect of Electrolyte
Type on the Electrophoretic Mobility of Polystyrene Latex Colloids.
Colloids and Surfaces 44:165-178.
Elimelech, M. and O'Melia, C.R. 1990. Kinetics of Deposition
of Colloidal Particles in Porous Media. Environ. Sci. Technol.
24:1528-1536.
Elimelech, M. 1990. Indirect Evidence of Hydration Forces in
the Deposition of Polystyrene Latex Colloids on Glass Surfaces.
J. Chem. Soc., Faraday Trans. 86(9):1623-1624.
Elimelech, M. 1991. Kinetics of Capture of Colloidal Particles
in Packed Beds under Attractive Double Layer Interactions. J.
Coll. Interface Sci. 146:337-352.
El-Deeb, S., Thompson, S.C., and Covault, J. 1992. Characterization
of a Cell Surface Adhesion Molecule Expressed by a Subset of
Developing Chick Neurons. Devel. Biol. 149:213-227.
Gallagher, P.D. 1991. The interaction of colloidal polystyrene
latex spheres with a critical binary liquid mixture. PH.D.
Thesis, University of Pittsburgh.
Gallagher, P.D. and Maher, J.V. 1992. Partition and migration
of polystyrene latex spheres in critical liquid mixtures. Phys.
Rev. A46:2012.
Gallagher, P.D., Kurnaz, M.L., and Maher, J.V. 1992. Aggregation
in polystyrene-sphere suspensions in near-critical binary liquid
mixtures. Phys. Rev. A46:7750.
Gombocz, E., Tietz, D., Hurt, S.S., and Chrambach, A. 1987.
Polystyrene Latex Particles as Size Standards in Quantitative
Agarose Gel Electrophoresis Application to Three Plant Viruses.
Electrophoresis 8:261-271.
Hague, M.F., Kallay, N., Privman, V., and Matijevic, E. 1990.
Magnetic Effects in Particle Adhesion. I. Kinetics of Hematite
Deposition on Stainless Steel. J. Coll. Interface Sci.
137:36-47.
Handa, K., Nudelman, E.D., Stroud, M.R., Shiozawa, T., and Hakomori,
S. 1991. Selectin GMP-140 (CD62-PADGEM) Binds to Sialosyl-Lea
and Sialosyl-Lex, and Sulfated Glycans Modulate this
Binding. Biochem. Biophys. Res. Commun. 181:1223-1230.
Johnson, C.J. 1995. Applications of Scanning Probe Microscopy
Pr.4: AFM Imaging in Fluids for the Study of Colloidal Particle
Adsorption. American Laboratory 12:12.
Kurnaz M.L., Gallagher, P.D., and Maher, J.V. 1992. Polystyrene
latex spheres in one phase region of a critical binary liquid
mixture. Abstract, Bulletin of the American Physical Society
37:393.
Lefern, C.K., Swinger, V.L., Kong, H.C., and Haughland, R.P.
1995. Quantitative Nonradioactive CAT Assays using Fluorescent
BODIPY 1-Deoxy chloramphonical Substances. Biotechniques
19, 488-493.
Olal, Andrew D.A. 1990. Surface and Colloid Chemical Study
of the Interaction of Proteins with Polystyrene Latex. Ph.
D. Thesis, Dept. Chemistry, UBC, May.
Perkins, T.T., Smith, D.E., and Chu, S. 1994. Direct Observation
of tube-like motion of a single polymer chain. Science
264:819-822.
Pouvelle, B., Spiegel, R., Hsiao, L., Howard, R.J., Morris,
R.L., Thomas, A.P., and Taraschi, T.F. 1991. Direct Access to
Serum Macromolecules by Intra-erythrocytic Malaria Parasites.
Nature 353:73-75.
Ranby, M., Johansson, G., and Smith, M. 1993. Rapid Latex Test
for Fibrin d-Dimer with Diagnostic Properties Comparable to
ELISA. XIVth Congress of the International Society on Thrombosis
and Hemostasis.
Seaman, G.V.F. and Goodwin, J.W. 1986. Physicochemical factors
in latex rapid agglutination tests. Amer. Clin. Prod. Rev.
June:25-31.
Serwer, P., and Hayes, S.J. 1986. Exclusion of Spheres by Agarose
Gels during Agarose Gel Electrophoresis: Dependence on the Sphere's
Radius and the Gel's Concentration. Anal. Biochem. 158:72-78.
Snyder, R.S., Rhodes, P.H., Miller, T.Y., Micale, F.J., Mann,
R.V., and Seaman, G.V.F. 1986. Polystyrene Latex Separations
by Continuous Flow Electrophoresis on the Space Shuttle. Sep.
Sci. Technol. 21:157-185.
Van Orman, B.B. and McIntire, G.L. 1989. Analytical Separation
of Polystyrene Nanospheres by Capillary Electrophoresis. J.
Microcolumn Sep. 1(6):289-293.
Wan, J. and Wilson, J.L. 1992. New findings on Particle Transport
Within the Vandose Zone: The Role of the Gas-Water Interface.
Proc. Conf. AGU Hydrology Days. Fort Collins, Colorado,
March-April.
Xu. R. 1996. Reference Materials in Particle Measurements in
"Liquid and Surface-borne Particle Measurement Handbook," Ed
Knapp, Barber and Liebman. Chapter 19, 709-720, Marcel Dekker,
NY.
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